Capillary　zone　electrophoresis　(CZE)　with　UV　detection　at　214nm　was　developed　for　the　simultaneous　determination　of　phosphate　and　calcium　in　waters,　where　Ca2+　reacted　with　2,6-pyridinedicarboxylic　acid　(2,6-PDCA)　in　the　electrolyte　to　form　anionic　Ca[PDCA]2-2.　Consequently,　HPO2-4　and　Ca[PDCA]2-2　were　simultaneously　separated　by　CZE　using　an　electrolyte　containing　10mM　2,6-PDCA　and　0.75mM　tetradecyltrimethylammonium　bromide　(TTAB)　at　pH　7.0.　The　results　showed　that　reasonable　resolution　with　low　interference　from　other　ions　in　the　water　was　achieved.　Linear　calibration　curves　were　obtained　in　the　concentration　range　of　0.01-0.5mM　with　hydrodynamic　injection.　The　detection　limits　(S/N=3)　were　5　μM　for　[Ca(PDCA)2]2-　and　2　μM　for　[HPO4]2-.　The　separation　of　[HPO4]2-　and　Ca[PDCA]2-2　occurred　within　6　min　with　small　relative　standard　deviation　of　peak　areas　(2-4　and　Ca2+　in　river　water　samples,　and　provided　a　fast　and　simple　procedure　that　required　no　modification　of　the　CE　instrument.　©　Taylor　&　Francis.