Dual　inhibition　of　glycolysis　and　oxidative　phosphorylation(OXPHOS)can　break　the　metabolic　plasticity　of　cancer　cells　to　inhibit　most　energy　supply　and　lead　to　effective　cancer　therapy.However,the　pharmacokinetic　difference　among　drugs　hinders　these　two　inhibitions　to　realize　a　uniform　temporal　and　spatial　distribution.Herein,we　report　an　aptamer-based　artificial　enzyme　for　simultaneous　dual　inhibition　of　glycolysis　and　OXPHOS,which　is　constructed　by　arginine　aptamer　modified　carbon-dots-doped　graphitic　carbon　nitride(AptCCN).AptCCN　can　circularly　capture　intracellular　arginine　attribute　to　the　specific　binding　ability　of　arginine　aptamers　to　arginine,and　further　catalyze　the　oxidation　of　enriched　arginine　to　nitric　oxide(NO)under　red　light　irradiation.In　vitro　and　in　vivo　experiments　showed　that　arginine　depletion　and　NO　stress　could　inhibit　glycolysis　and　OXPHOS,leading　to　energy　blockage　and　apoptosis　of　cancer　cells.The　presented　aptamer-based　artificial　enzyme　strategy　provides　a　new　path　for　cell　pathway　regulation　and　synergistic　cancer　therapy.