Many　methods　have　been　used　to　detect　heavy　metals　in　herbal　medicines,　while　few　are　developed　to　remove　them.　In　this　study,　a　novel　genetically　engineered　fusion　protein　composed　of　metallothionein　(MT),　cellulose　binding　module　(CBM),　and　superfolder　GFP　(sfGFP)　was　designed　to　remove　heavy　metals.　MT,　a　kind　of　cysteine-rich　protein,　was　used　to　chelate　heavy　metals　with　high　specific　affinity.　The　CBM　facilitated　the　fusion　protein　MT-CBM-sfGFP　binding　to　cellulose　specifically,　which　made　the　purification　and　immobilization　in　one　step.　The　sfGFP　was　used　to　detect　the　fusion　protein　MT-CBM-sfGFP　easily　during　the　process　of　expression　and　immobilization.　The　MT　from　Cancer　pagurus　(MTCap)　and　the　CBM　from　Cellulomonas　fimi　(CBMcef)　were　used　as　an　example　and　the　fusion　protein　(MTCap-CBMcef-sfGFP)　was　expressed　in　Escherichia　coli.　Then,　the　cell　lysates　were　mechanically　mixed　with　cellulose　to　create　biosorbent　MTCap-CBMcef-sfGFP@cellulose.　The　efficiency　of　the　biosorbent　MTCap-CBMcef-sfGFP@cellulose　for　Pb2+　removal　was　evaluated　using　the　water　decoction　of　Honeysuckle　as　a　model.　Results　suggested　that　MTCap-CBMcef-sfGFP@cellulose　had　high　efficiency　for　Pb2+　removal　from　the　water　decoction　of　Honeysuckle　without　affecting　its　active　ingredients.　The　low-cost,　easy　production,　and　high　efficiency　of　the　biosorbent　enable　it　to　have　many　applications　in　heavy　metal　removal　from　aqueous　solutions　of　herbal　medicines　and　food.