The　integrity　of　tissue　is　crucial　for　a　high-quality　analysis　of　matrix-assisted　laser　desorption　ionization-mass　spectrometry　image　(MALDI-MSI).　Various　embedding　media　utilized　in　traditional　tissue-sectioning　techniques　are　generally　not　recommended　for　MALDI-MSI　of　dry　and　fragile　plant　tissues　because　of　the　ion　suppression　effect　in　MALDI　ionization　in　the　low-mass　region　as　well　as　the　undesirable　structural　deformation　during　the　sample　preparation.　In　this　work,　a　novel　poly-L-lysine　(PLL)-based　tissue　embedding　method　was　developed　for　MALDI-MSI　analysis　of　dry　and　fragile　aristolochia　plant　(AP)　tissues.　The　practical　application　in　fixation,　embedding,　cryosectioning,　and　mounting　of　the　dry　and　fragile　AP　tissues　demonstrated　that　the　PLL-based　embedding　technique　could　provide　good　rigidity　to　the　plant　tissues　analysis　compared　to　that　without　embedding　and　gelatin　embedding.　With　the　assistance　of　the　PLL　embedding　medium,　high　spatial　resolution　molecular　ion　maps　of　main　compounds,　including　aristolochic　acids　I　(AAI)　and　aristolochic　acids　II　(AAII)　in　AP　root　tissue,　could　be　achieved　by　MALDI-MSI　with　enhanced　signal　intensities　and　no　obvious　background　interference.　This　work　provides　an　alternative　approach　for　embedding　the　dry　and　fragile　plant　tissues　comparable　with　MALDI-MSI　analysis.　(C)　2019　Elsevier　B.V.　All　rights　reserved.