Whole-grain　food　(WGF)　is　well　known　for　its　anti-diabetic　effect,　and　alleviation　of　obesity-induced　insulin　resistance　(IR)　might　be　one　of　the　underlying　mechanisms.　In　the　current　study,　the　effects　of　starch,　as　the　main　component　in　WGF,　and　beta-glucan　in　a　whole-grain-like　structural　form　(WGLSF)　on　hepatic　IR　and　glucose　homeostasis　were　investigated　using　high-fat　(HF)-induced　obese　C57BL/6J　mice.　After　treatment　for　8　weeks,　the　body　weight　gain　and　IR　of　the　mice　were　significantly　reduced.　The　hepatic　Akt,　the　key　component　in　insulin　signaling,　was　activated,　and　the　hepatic　expression　and　protein　levels　of　glucose-6-phosphatase　(G-6-P)　and　phosphoenolpyruvate　carboxykinase　(PEPCK)　were　reduced.　Moreover,　WGLSF　effectively　reduced　the　hepatic　levels　of　free　fatty　acids　and　the　pro-inflammatory　cytokines　TNF-alpha,　IL-6,　and　NF-kappa　B.　Additionally,　the　reduced　level　of　the　phosphorylated　c-Jun-N-terminal　kinases　(JNK)　indicated　that　WGLSF　treatment　might　inactivate　the　JNK　signaling,　leading　to　improved　hepatic　IR.　These　results　demonstrated　that　starch　and　beta-glucan　in　a　whole　grain-like　structural　form　have　the　potential　as　a　dietary　strategy　to　combat　obesity-induced　hepatic　IR　for　improved　health.