A　novel　method　of　atmospheric　pressure　gas　chromatography-tandem　mass　spectrometry　(APGC-MS/MS)　for　the　analysis　of　organochlorine　pesticides　(OCPs)　in　human　serum　was　developed.　Better　sensitivity　and　selectivity　were　achieved　compared　to　conventional　gas　chromatography-mass　spectrometry　(GC-MS)　and　GC-MS/MS.　The　limits　of　detection　(LODs)　of　OCPs　ranged　from　0.22　to　0.90　pg/mL　for　the　analysis　in　serum　samples.　The　recoveries　and　repeatability　of　the　developed　method　were　evaluated　from　the　analysis　of　spiked　serum　at　three　levels　(0.05,　0.5,　and　5　ng/mL).　The　recoveries　ranged　from　84.5　to　118.2%,　and　the　relative　standard　deviations　(RSDs)　were　less　than　20%.　The　developed　APGC-MS/MS　method　was　applied　to　measure　OCPs　in　pooled　human　serum　from　the　interlaboratory　comparison　study.　The　obtained　results　of　OCPs　were　within　the　acceptable　ranges.　The　method　has　been　successfully　applied　to　the　analysis　of　OCPs　in　human　serum　samples　with　the　sample　volume　of　up　to　100　mu　L.