In　this　paper,　the　new　configuration　of　transcritical　CO2　refrigeration　cycle　combined　with　a　thermoelectric　subcooler　and　an　ejector　(TES　+　EJE)　is　proposed.　The　thermoelectric　subcooler　is　installed　after　the　gas　cooler　in　the　transcritical　CO2　refrigeration　cycle　with　an　ejector.　Comparisons　are　carried　out　with　the　conventional　transcritical　CO2　refrigeration　cycle　(BASE),　CO2　cycle　with　a　thermoelectric　subcooler　(TES)　and　CO2　cycle　with　an　ejector　(EJE).　Maximum　cooling　coefficient　of　performance　(COPc)　is　obtained　for　the　new　TES　+　EJE　cycle　with　a　simultaneous　optimization　of　subcooling　temperature　and　discharge　pressure.　The　improved　new　cycle　exhibits　higher　COPc　and　lower　discharge　pressure　compared　with　the　other　three　cycles.　Compared　with　the　BASE　cycle,　the　maximum　COPc　of　the　TES　+　EJE　cycle　is　increased　by　39.34%　and　the　corresponding　optimum　discharge　pressure　is　reduced　by　8.01%　under　given　operation　conditions　of　5　degrees　C　evaporation　temperature　and　40　degrees　C　gas　cooler　outlet　temperature.　The　effects　of　the　subcooling　temperature,　discharge　pressure,　gas　cooler　outlet　temperature　and　evaporation　temperature　on　the　TES　+　EJE　system　performance　are　also　discussed　by　energy　and　exergy　analysis　and　results　present　here.