The　size　of　particles　is　a　principal　parameter　to　design　drug　delivery　vehicles.　In　this　work,　albumin　nanoparticles　were　fabricated　from　microscaled　poly[lactic-co-(glycolic　acid)]　(PLGA)　templates　using　layer-by-layer　(LbL)　assembly,　which　are　different　from　traditional　LbL-based　hollow　capsules.　The　albumin　nanoparticles　exhibit　a　spherical　shape　and　solid　inner　structure.　The　formation　mechanism　of　these　albumin　nanoparticles　was　proposed　based　on　PLGA　templates,　mechanical　property　of　albumin　and　external　sonication.