Biomimetic　mineralized　hybrid　scaffolds　are　widely　used　as　natural　bone　substitute　materials　in　tissue　engineering　by　mimicking　vital　characters　of　extracellular　matrix　(ECM).　However,　the　fabrication　of　hybrid　scaffolds　with　suitable　mechanical　properties　and　good　biocompatibility　remains　a　challenge.　To　solve　the　problems　mentioned　above,　biomimetic　calcium　phosphate　mineralized　organic-inorganic　hybrid　scaffold　composed　of　nano　hydroxyapatite　(nHAP),　Chitosan　(CS),　Chondroitin　sulfate　(CSA)　and　hyaluronic　acid　(HA)　with　hierarchical　micro/nano　structures　was　successfully　developed.　In　this　process,　an　efficient　and　easy-to-accomplish　method　combining　in　situ　biomimetic　synthesis　with　freeze-drying　technology　was　applied.　The　chemical　structure　of　the　scaffolds　was　confirmed　by　Fourier　transform　infrared　spectroscopy　(FTIR)　and　X-ray　diffraction　(XRD).　Surface　morphology　of　scaffolds　was　characterized　by　Scanning　electron　microscopy　(SEM).　The　nHAP/CS/CSA/HA　hybrid　scaffolds　with　a　well　distributed　pore　size　showed　suitable　mechanical　strength　which　is　not　only　due　to　the　addition　of　the　nHAP　but　also　the　interaction　between　the　positively　charged　CS　and　the　negatively　charged　CSA　and　HA.　Simultaneously,　the　biocompatibility　was　evaluated　by　the　MTT　cytotoxicity　assay,　alkaline　phosphatase　(ALP)　activity,　Hoechst　33258　fluorescence　staining.　All　those　results　proved　that　the　scaffolds　possess　good　biocompatibility　and　the　components　added　have　enhanced　the　proliferation　and　differentiation　of　osteoblast.　Thus,　it　can　be　anticipated　that　the　in　situ　biomimetic　mineralized　nHAP/CS/CAS/HA　hybrid　scaffolds　will　be　promising　candidates　for　bone　tissue　engineering.　(C)　2017　Elsevier　B.V.　All　rights　reserved.