A　＂two-step＂　pressurized　microwave-assisted　extraction　method　coupled　with　ion　chromatography　with　inductively　coupled　plasma　mass　spectrometry　for　the　determination　of　different　arsenic　species　in　spirulina　samples　was　developed.　The　extraction　method　used　H2O2/H2O　(1:　5,　v/v)　as　solvent　to　extract　all　arsenic　species　except　arsenite,　which　was　extracted　by　using　water　as　solvent.　The　extraction　method　had　a　satisfactory　recovery　(＞96%)　and　took　a　short　time　(20.0　min).　With　our　method,　all　arsenic　species　in　spirulina　samples　were　completely　separated　and　determined　with　recoveries　of　84-105%　and　relative　standard　deviations　of　2-4%.　Food-grade　spirulina　powder　samples　from　seven　provinces　(Inner　Mongolia,　Zhejiang,　Fujian,　Hainan,　Yunnan,　Jiangsu,　and　Guangxi)　in　China　were　analyzed　using　the　optimized　protocol.　Arsenate　was　detected　at　the　concentration　range　of　170-394　ng/g　in　all　the　spirulina　samples.　Dimethylarsinic　acidwas　detected　at　the　concentration　range　of　32839　ng/g　in　spirulina　from　above-six　provinces　except　Guangxi.　Monomethylarsonic　acid　(67　+/-　3　ng/g)　was　detected　only　in　spirulina　from　Yunnan　province.　Arsenite　was　detected　at　the　concentration　range　of　28-147　ng/g　in　spirulina　from　above　five　provinces　except　Hainan　and　Guangxi.　Five　unknown　organic　arsenic　species　were　found　in　spirulina　from　above　six　provinces　except　Guangxi.