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author:

Xu, Jianguo (Xu, Jianguo.) [1] | Wu, Zai-Sheng (Wu, Zai-Sheng.) [2] (Scholars:吴再生) | Shen, Weiyu (Shen, Weiyu.) [3] | Le, Jingqing (Le, Jingqing.) [4] | Zheng, Tingling (Zheng, Tingling.) [5] | Li, Hongling (Li, Hongling.) [6] | Jia, Lee (Jia, Lee.) [7]

Indexed by:

EI Scopus SCIE

Abstract:

As one of the most exciting building blocks, DNA has gained increasing attention in the construction of promising nanostructures for various biological and medical purposes. In this contribution, we have developed an easily constructed DNA nanoassembly-based biosensing system that consists of one signal hairpin probe (SHP) and one label -free hairpin probe (LHP) for target p53 gene analysis. The probes of SHP and LHP were designed to be incapable of interacting with each other in the absence of the p53 gene. When the target gene is introduced, the 3' end of SHP (or LHP) hybridizes with the middle region of LHP (or SHP), leading to polymerase-sustained DNA nanoassembly. Because one target species can exhaust many building scaffolds to execute the programmable nanoassembly in one-pot approach, the fluorescence intensity of SHP is greatly enhanced in the presence of target gene in a simple and robust manner. The practical applicability was successfully demonstrated by screening target gene extracted from cancer cells. We believe this intriguing sensing strategy and desirable assay ability would provide new opportunities to develop versatile biochemical analysis methods.

Keyword:

Biosensing DNA nanoassembly p53 gene

Community:

  • [ 1 ] [Xu, Jianguo]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 2 ] [Wu, Zai-Sheng]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 3 ] [Shen, Weiyu]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 4 ] [Le, Jingqing]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 5 ] [Zheng, Tingling]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 6 ] [Li, Hongling]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 7 ] [Jia, Lee]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Canc Metastasis Alert & Prevent Ctr, Fuzhou 350116, Peoples R China
  • [ 8 ] [Xu, Jianguo]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 9 ] [Wu, Zai-Sheng]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 10 ] [Shen, Weiyu]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 11 ] [Le, Jingqing]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 12 ] [Zheng, Tingling]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 13 ] [Li, Hongling]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 14 ] [Jia, Lee]Fuzhou Univ, State Key Lab Photocatalysis Energy & Environm, Biopharmaceut Photocatalysis, Fuzhou 350116, Peoples R China
  • [ 15 ] [Xu, Jianguo]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China
  • [ 16 ] [Wu, Zai-Sheng]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China
  • [ 17 ] [Shen, Weiyu]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China
  • [ 18 ] [Le, Jingqing]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China
  • [ 19 ] [Zheng, Tingling]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China
  • [ 20 ] [Li, Hongling]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China
  • [ 21 ] [Jia, Lee]Fuzhou Univ, Fujian Prov Key Lab Canc Metastasis Chemoprevent, Fuzhou 350116, Peoples R China

Reprint 's Address:

  • 吴再生

    [Wu, Zai-Sheng]Fuzhou Univ, CMAPC, Fuzhou 350002, Fujian, Peoples R China

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Related Keywords:

Source :

BIOSENSORS & BIOELECTRONICS

ISSN: 0956-5663

Year: 2017

Volume: 94

Page: 626-631

8 . 1 7 3

JCR@2017

1 0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:226

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 23

SCOPUS Cited Count: 23

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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