Wound　treatment　should　meet　the　challenge　both　of　preventing　infection　and　promoting　wound　healing.　To　design　a　sequential　delivery　system　for　wound　healing,　PLGA-glycol　chitosan　(GC)　core-shell　micro　spheres　containing　chlorhexidine　acetate　(CHA)　at　the　GC　shell　and　bFGF　in　the　core　of　PLGA　microspheres　were　fabricated　using　emulsion-solvent　evaporation　method.　SEM　showed　that　the　microspheres　were　all　spherical　in　shape　with　a　smooth　surface.　The　average　size　of　PLGA-GC　microspheres　increased　due　to　the　GC　coating　on　the　surface.　The　results　of　release　profiles　and　fluorescence　images　indicated　that　PLGA-GC　microspheres　had　an　ability　to　deliver　drugs　in　sequence.　The　CHA　was　rapidly　released,　whereas　the　proteins　presented　a　sustained　release.　The　release　behavior　could　be　modulated　by　changing　the　GC　amount.　Antibacterial　assay　and　cell　proliferation　tests　suggested　that　the　released　CHA　and　bFGF　retained　their　antimicrobial　activity　and　bioactivity　during　preparation.　The　microspheres　exhibited　non-cytotoxicity　against　3T3　cells　and　had　a　good　biocompatibility.　These　results　demonstrated　that　PLGA-GC　core-shell　microspheres　could　be　a　promising　controlled　release　system　of　delivering　drugs　and　proteins　in　sequence　for　wound　healing.　(C)　2016　Elsevier　B.V.　All　rights　reserved.