Indexed by:
Abstract:
A versatile enzyme-free deoxyribonucleic acid (DNA) detection method based on hybridization chain reaction (HCR) and fluorescence resonance energy transfer (FRET) was developed. Hairpin H1 and H2 are labelled with carboxyfluorescein (FAM) as the donor and tetramethylrhodamine (TAMRA) as the acceptor, respectively. In the presence of target DNA, long nicked dsDNA nanowires are self-assembled through HCR process. The donor and the acceptor are brought in close proximity, resulting in a FRET process between them. The ratio of fluorescent intensities of the acceptor and donor can be used to quantitatively detect the target DNA with a limit of detection 0.7 nmol L-1. This proposed method was applied to detect DNA in biologic samples with satisfactory results. (C) 2016 Elsevier B.V. All rights reserved.
Keyword:
Reprint 's Address:
Email:
Version:
Source :
SENSORS AND ACTUATORS B-CHEMICAL
ISSN: 0925-4005
Year: 2016
Volume: 233
Page: 691-696
5 . 4 0 1
JCR@2016
8 . 0 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
ESI HC Threshold:235
JCR Journal Grade:1
CAS Journal Grade:1
Cited Count:
WoS CC Cited Count: 35
SCOPUS Cited Count: 34
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 3
Affiliated Colleges: