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author:

Chen, Ying (Chen, Ying.) [1] | Chen, Ling (Chen, Ling.) [2] | Ou, Yidian (Ou, Yidian.) [3] | Guo, Liangqia (Guo, Liangqia.) [4] (Scholars:郭良洽) | Fu, Fengfu (Fu, Fengfu.) [5] (Scholars:付凤富)

Indexed by:

EI Scopus SCIE

Abstract:

A versatile enzyme-free deoxyribonucleic acid (DNA) detection method based on hybridization chain reaction (HCR) and fluorescence resonance energy transfer (FRET) was developed. Hairpin H1 and H2 are labelled with carboxyfluorescein (FAM) as the donor and tetramethylrhodamine (TAMRA) as the acceptor, respectively. In the presence of target DNA, long nicked dsDNA nanowires are self-assembled through HCR process. The donor and the acceptor are brought in close proximity, resulting in a FRET process between them. The ratio of fluorescent intensities of the acceptor and donor can be used to quantitatively detect the target DNA with a limit of detection 0.7 nmol L-1. This proposed method was applied to detect DNA in biologic samples with satisfactory results. (C) 2016 Elsevier B.V. All rights reserved.

Keyword:

DNA Fluorescence resonance energy transfer Hybridization chain reaction Ratiometric measurement

Community:

  • [ 1 ] [Chen, Ying]Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China
  • [ 2 ] [Chen, Ling]Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China
  • [ 3 ] [Ou, Yidian]Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China
  • [ 4 ] [Guo, Liangqia]Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China
  • [ 5 ] [Fu, Fengfu]Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China

Reprint 's Address:

  • 郭良洽

    [Guo, Liangqia]Fuzhou Univ, Coll Chem, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350116, Fujian, Peoples R China

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Source :

SENSORS AND ACTUATORS B-CHEMICAL

ISSN: 0925-4005

Year: 2016

Volume: 233

Page: 691-696

5 . 4 0 1

JCR@2016

8 . 0 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:235

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 35

SCOPUS Cited Count: 34

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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