In　this　work,　we　demonstrate　for　the　first　time,　a　method　to　synthesize　phenylboronic　acid-Fe3O4@polydopamine　(Fe3O4@PDA-PBA)　magnetic　microspheres　via　the　combination　of　mussel-inspired　polydopamine　coating　and　click　chemistry.　Uniform-size　and　core-shell　structured　Fe3O4@PDA-PBA　magnetic　microspheres　with　a　core　diameter　of　similar　to　240　nm　and　a　shell　thickness　of　similar　to　13　nm　were　obtained　as　identified　by　the　characterization　of　the　morphology,　structure　and　composition　of　the　synthesized　microspheres.　We　evaluated　the　selectivity　and　binding　capacity　of　the　Fe3O4@PDA-PBA　magnetic　microspheres　by　using　standard　glycoproteins　(ovalbumin,　immunoglobulin　G　and　catalase)　and　nonglycoproteins　(human　serum　albumin,　bovine　hemoglobin,　myoglobin,　lysozyme,　and　ribonuclease　A)　as　model　proteins.　Adsorption　experiments,　SDS-PAGE　and　mass　spectrometry　analysis　demonstrated　that　the　Fe3O4@PDA-PBA　magnetic　microspheres　had　much　high　binding　capacity　and　selectivity　for　glycoproteins/glycopeptides　compared　to　nonglycoproteins/nonglycopeptides.　In　addition,　the　practicability　of　the　Fe3O4@PDA-PBA　magnetic　microspheres　was　further　assessed　by　selective　capture　of　glycoproteins　from　healthy　human　serum.　The　good　results　demonstrated　its　potential　in　glycoproteome　analysis.