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author:

Wang, Guozeng (Wang, Guozeng.) [1] (Scholars:王国增) | Huang, Xiaoyun (Huang, Xiaoyun.) [2] | Ng, Tzi Bun (Ng, Tzi Bun.) [3] | Lin, Juan (Lin, Juan.) [4] (Scholars:林娟) | Ye, Xiu Yun (Ye, Xiu Yun.) [5] (Scholars:叶秀云)

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Scopus SCIE

Abstract:

Soda lakes are one of the most stable naturally occurring alkaline and saline environments, which harbor abundant microorganisms with diverse functions. In this study, culture-independent molecular methods were used to explore the genetic diversity of glycoside hydrolase (GH) family 10 and GH11 xylanases in Lake Dabusu, a soda lake with a pH value of 10.2 and salinity of 10.1%. A total of 671 xylanase gene fragments were obtained, representing 78 distinct GH10 and 28 GH11 gene fragments respectively, with most of them having low homology with known sequences. Phylogenetic analysis revealed that the GH10 xylanase sequences mainly belonged to Bacteroidetes, Proteobacteria, Actinobacteria, Firmicutes and Verrucomicrobia, while the GH11 sequences mainly consisted of Actinobacteria, Firmicutes and Fungi. A full-length GH10 xylanase gene (xynAS10-66) was directly cloned and expressed in Escherichia coli, and the recombinant enzymes showed high activity at alkaline pH. These results suggest that xylanase gene diversity within Lake Dabusu is high and that most of the identified genes might be novel, indicating great potential for applications in industry and agriculture.

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Community:

  • [ 1 ] [Wang, Guozeng]Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China
  • [ 2 ] [Huang, Xiaoyun]Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China
  • [ 3 ] [Lin, Juan]Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China
  • [ 4 ] [Ye, Xiu Yun]Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China
  • [ 5 ] [Wang, Guozeng]Natl Engn Lab High Efficiency Enzyme Express, Fuzhou 350002, Peoples R China
  • [ 6 ] [Lin, Juan]Natl Engn Lab High Efficiency Enzyme Express, Fuzhou 350002, Peoples R China
  • [ 7 ] [Ye, Xiu Yun]Natl Engn Lab High Efficiency Enzyme Express, Fuzhou 350002, Peoples R China
  • [ 8 ] [Ng, Tzi Bun]Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Shatin, Hong Kong, Peoples R China

Reprint 's Address:

  • 林娟

    [Lin, Juan]Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China

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Source :

PLOS ONE

ISSN: 1932-6203

Year: 2014

Issue: 11

Volume: 9

3 . 2 3 4

JCR@2014

2 . 9 0 0

JCR@2023

ESI Discipline: MULTIDISCIPLINARY;

ESI HC Threshold:320

JCR Journal Grade:1

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count: 76

SCOPUS Cited Count: 13

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Online/Total:177/9999050
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