This　paper　reports　the　electrochemical　deposit　and　characterization　of　copper　nanoparticles　on　the　multi-walled　carbon　nano-tubes　(MWCNTs)　and　the　application　of　nano-copper-MWCNT-modified　glassy　carbon　electrode　(GCE)　in　the　electrochemical　determination　of　dopamine　(DA).　Cyclic　voltammetric　experiments　indicated　that　the　nano-copper-MWCNT-modified　GCE　displayed　very　high　electrochemical　catalytic　activity　compared　to　the　MWCNT-modified　GCE　in　0.06　M　H2SO4.　The　nano-copper-MWCNT-modified　GCE　was　found　to　be　suitable　for　the　selective　determination　of　DA.　The　presence　of　uric　acid　(UA)　and　high　concentration　ascorbic　acid　(AA)　did　not　interfere　in　the　detection　of　DA　on　this　electrode.　The　observed　linear　range　of　the　glassy　carbon　electrode　modified　by　nano-copper-MWCNT　composite　membrane　was　from　0.1　to　156.0　mu　M　for　DA　and　its　detection　limit　was　0.05　mu　M.　It　was　demonstrated　that　this　electrode　can　be　used　for　the　determination　of　DA　in　real　samples　such　as　DA　injectable　formulations　and　human　urine.