A　novel　method　of　microemulsion　electrokinetic　capillary　chromatography　(MEEKC)　coupled　with　on-line　large　volume　sample　stacking　was　developed　for　the　analysis　of　six　plant　hormones　including　indole-3-acetic　acid,　indole-3-butyric　acid,　indole-3-propionic　acid,　1-naphthaleneacetic　acid,　abscisic　acid　and　salicylic　acid.　Baseline　separation　of　six　plant　hormones　was　achieved　within　10　min　by　using　the　microemulsion　background　electrolyte　containing　a　97.2%　(w/w)　10　mM　borate　buffer　at　pH　9.2,　1.0%　(w/w)　ethyl　acetate　as　oil　droplets,　0.6%　(w/w)　sodium　dodecyl　sulphate　as　surfactant　and　1.2%　(w/w)　1-butanol　as　cosurfactant.　In　addition,　an　on-line　concentration　method　based　on　a　large　volume　sample　stacking　technique　and　multiple　wavelength　detection　was　adopted　for　improving　the　detection　sensitivity　in　order　to　determine　trace　level　hormones　in　a　real　sample.　The　optimal　method　provided　about　50-100　fold　increase　in　detection　sensitivity　compared　with　a　single　MEEKC　method,　and　the　detection　limits　(S/N　=　3)　were　between　0.005　and　0.02　mu　g　mL(-1).　The　proposed　method　was　simple,　rapid　and　sensitive　and　could　be　applied　to　the　determination　of　six　plant　hormones　in　spiked　water　samples,　tobacco　leaves　and　1-naphthylacetic　acid　in　leaf　fertilizer.　The　recoveries　ranged　from　76.0%　to　119.1%,　and　good　reproducibilities　were　obtained　with　relative　standard　deviations　(RSDs)　less　than　6.6%.