A　sensitive　method　based　on　capillary　electrophoresis(CE)　with　end-column　amperometric　detection(　AD)　has　been　developed　for　the　chiral　separation　and　determination　of　sotalol　enantiomers.　Factors　affecting　the　chiral　CE　separation　of　the　analytes,　such　as　working　potential,　acidity,　concentration　of　running　buffer　and　the　chiral　selector,　injection　and　applied　voltage,　were　extensively　investigated.　The　working　electrode　operated　in　the　wall-jet　configuration　was　a　carbon　disk　electrode(vs.　Ag/AgCl).　Under　the　optimum　conditions,　baseline　separation　of　the　enantiomers　could　be　accomplished,　and　excellent　linearity　was　observed　between　peak　current　and　concentration　of　enantiomers　in　the　range　from　5　mu　g/L　to　500　mu　g/L.　The　limits　of　detection(S/N　=　3)　were　2.0　and　1.9　mu　g/L,　respectively.　The　proposed　method　has　been　applied　to　the　analysis　of　sotalol　in　human　serum　samples　with　satisfactory　results.