Central　nervous　system　disorders,　including　cerebrovascular　disease,　neurodegenerative　diseases　and　head　trauma　are　the　most　common　cause　of　severe　disability　in　adults　and　share　a　number　of　pathophysiological　features.　The　therapeutic　strategy　of　neuroprotection　has　been　well　accepted　as　one　of　the　promising　approaches　in　treating　such　brain　disorders,　and　searching　for　the　effective　neuroprotective　agents　is　still　an　open-ended　task　for　neurologists　and　neuro-pharmacologists.　In　this　study,　we　report　for　the　first　time　that　the　enzymatic　hydrolysates　from　type-B　porcine　hide　gelatin　has　potent　neuroprotective　activity　against　H2O2-　or　serum　deprivation-induced　injuries　of　cultured　SH-SY5Y　cells.　The　peptides　used　in　this　study　were　prepared　from　type-B　porcine　hide　gelatin　digested　with　pepsin　and　papain.　The　neuroprotective　activity　of　the　porcine　hide　gelatin　hydrolysate　(PHH)　was　evaluated　using　MTT　reduction　assay.　From　the　pre-screening　of　PHH,　we　found　that　the　whole　porcine　hide　gelatin　hydrolysate　obtained　from　papain　digestion　(PHH-I)　showed　significant　neuroprotective　activities　(P＜0.05).　After　further　separation　of　PPH-I　through　SP-Sephadex　C-50　and　Sephadex　G-25,　only　the　fraction　with　smaller　molecular　weight　from　Sephadex　G-25　(PHH-Ic)　demonstrated　potent　neuroprotective　activities　(P＜0.01).　The　active　fraction　showed　a　molecular　mass　between　1,000-3,000Da　in　SDS-polyacrylamide　gel　electrophoresis,　and　was　rich　in　Glycine,　Proline　and　Hydroxyproline　in　amino　acid　composition,　indicating　that　peptides　with　a　spectrum　of　molecular　sizes　and　certain　amino　acids　are　critical　for　the　neuroprotective　activities　of　gelatin　peptides.　The　viability　of　cultured　cells　treated　with　gelatin　peptides　was　significantly　improved　in　a　dose-dependent　manner.　Further　studies　are　necessary　to　establish　the　neuroprotective　activity　of　hydrolyzed　peptides　for　the　neurons　in　vivo.