A　non-specific　lipid　transfer　peptide　(nsLTP)　with　antimicrobial　activity　was　isolated　from　the　mung　bean　(Phaseolus　mungo)　seeds.　The　procedure　entailed　aqueous　extraction,　ion　exchange　chromatography　on　CM-Sephadex　and　high　performance　liquid　chromatography　(HPLC)　on　POROS-HS-20.　The　peptide　exhibited　a　molecular　mass　of　9.03　kDa　in　mass　spectrometry.　It　exerted　antifungal　action　toward　Fusarium　solani,　Fusarium　oxysporum,　Pythium　aphanidermatum　and　Sclerotium　rolfsii,　and　antibacterial　action　against　Staphylococcus　aureus　but　not　against　Salmonella　typhimurium.　The　lipid　binding　of　this　peptide　was　very　similar　to　that　of　a　previously　described　lipid　transfer　protein　extracted　from　wheat　seeds　and　maize　seeds,　indicating　that　it　possessed　lipid　transfer　activity.　The　present　findings　add　to　the　scarcity　of　the　literature　on　leguminous　nsLTPs.　(C)　2004　Elsevier　Inc.　All　rights　reserved.