The　objective　of　this　study　was　to　understand　the　effect　of　biomass　concentration　(BC),　milling　times　(MT)　and　volumetric　hexane　to　water　ratio　(HWR)　on　lipid　recovery　rate　of　microalgae　Scenedesmus　dimorphus　in　the　wet　milling　process.　A　second-order　response　surface　regression　model　(R-2＞95%)　was　developed　based　on　a　three-variable　five-level　experiment　using　the　Central　Composite　Design　method.　Modeling　results　indicated　that　HWR　(Pr＜0.001)　and　BC　(Pr＜0.01)　significantly　affected　lipid　recovery　rate.　The　optimum　settings　were　found　to　be　21.2　g　l(-1)　BC,　4　MT　and　0.70　HWR,　at　which　98.5%　of　lipids　were　recovered.　The　results　indicated　that　the　wet　grinding　mill　was　effective　in　lipid　recovery　from　S.　dimorphus.