A　comprehensive　study　of　transportation　features　of　nitrogen,　phosphorous　and　dissolve　oxygen　at　the　sediment-water　interface　was　carried　out　in　near　city　areas　of　east　Lake　Chaohu.　Based　on　the　investigation　of　sediment　nutrients　analysis　and　nutrients　release　incubation　experiments,　the　release　potential　and　release　rates　of　ammonia　nitrogen　(NH3-N)　and　soluble　reactive　phosphorous　(SRP)　of　different　sediments　were　estimated.　The　distribution　characteristics,　diffusion　fluxes,　and　consumption　rates　of　dissolved　oxygen　(DO)　at　the　sediment-water　interface　of　different　sediments　were　studied　by　using　a　non-invasive　microelectrode　analysis　system.　The　results　showed　the　surface　sediments　in　the　near　city　areas　of　east　Lake　Chaohu　were　in　severe　nitrogen　and　phosphorous　pollution　with　high　total　nitrogen　(TN)　and　total　phosphorus　(TP)　loadings.　Typical　internal　releases　of　NH3-N　and　SRP　were　observed　from　all　sediments　with　the　average　release　rates　of　32.44　mg/(m2•d)　and　1.25　mg/(m2•d),　respectively.　The　sediments　play　the　role　of　pollution　source　rather　than　the　sink　for　NH3-N　and　SRP　in　the　study　area.　Results　also　showed　that　all　overlying　water　were　at　aerobic　condition.　The　average　oxygen　penetration　depth　(OPD)　and　oxygen　diffusion　flux　at　sediment-water　micro-interfaces　reached　5.3　mm　and　4.56　mmol/(m2•d)　respectively,　indicating　good　DO　diffusion　ability　from　the　boundary　layer　to　the　sediment.　The　internal　release　intensity　of　NH3-N　and　SRP　are　influenced　by　TN/TP　contents　of　the　surface　sediment　as　well　as　the　OPD　at　the　sediment-water　interface.　The　release　of　NH3-N　and　SRP　from　sediments　could　be　benefited　from　the　high　nitrogen　and　phosphorous　loadings　and　low　OPD　and　contribute　to　the　eutrophication　of　the　lake.　©　2020　by　Journal　of　Lake　Sciences.