Construction of a Target-Initiated, Enzyme-Free DNA Cascade Circuit for Amplified Detection of Mercury - Details

author：

Xing, Chao (Xing, Chao.) ^[1] | Chen, Ziyi (Chen, Ziyi.) ^[2] | Zhang, Cheng (Zhang, Cheng.) ^[3] | Wang, Jun (Wang, Jun.) ^[4] | Lu, Chunhua (Lu, Chunhua.) ^[5] (Scholars：卢春华)

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EI Scopus

Abstract：

Here,　we　designed　a　target-initiated,　enzyme-free　DNA　cascade　circuit　for　highly　selective　and　sensitive　detection　of　Hg2+　with　dual　amplification　based　on　concatenated　fuel　stimulant　powered　(FSP)　and　catalytic　hairpin　assembly　(CHA)　circuits.　In　a　typical　FSP-CHA　procedure,　in　the　presence　of　Hg2+,　the　invading　DNA　activated　the　upstream　FSP　circuit　to　release　the　hidden　strands　containing　multiple　trigger　units,　which　subsequently　motivated　the　downstream　CHA　circuit　to　drive　the　cross-opening　of　two　hairpin　substrates,　generating　tremendously　amplified　fluorescence　signals.　A　good　linearity　between　the　fluorescence　intensity　and　Hg2+　concentrations　was　observed　in　the　range　from　0.2　nM　to　20　nM.　The　proposed　system　showed　great　applicability　for　sensing　Hg2+　in　environmentally　relevant　water　samples.　Copyright　©　2020　American　Chemical　Society.

Keyword：

DNA Enzymes Fluorescence Mercury (metal) Rivers Timing circuits

Community：

[ 1 ] [Xing, Chao]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, Minjiang University, Fuzhou; 350108, China
[ 2 ] [Chen, Ziyi]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; 350116, China
[ 3 ] [Zhang, Cheng]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, Minjiang University, Fuzhou; 350108, China
[ 4 ] [Wang, Jun]Fujian Provincial Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, Minjiang University, Fuzhou; 350108, China
[ 5 ] [Lu, Chunhua]MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; 350116, China

Reprint 's Address：

[xing, chao]fujian provincial key laboratory of functional marine sensing materials, center for advanced marine materials and smart sensors, minjiang university, fuzhou; 350108, china

Email：

chaoxing@mju.edu.cn

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Version：

Construction of a Target-Initiated, Enzyme-Free DNA Cascade Circuit for Amplified Detection of Mercury
2020，ACS Applied Bio Materials

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Source ：

ACS Applied Bio Materials

ISSN： 2576-6422

Year： 2020

Issue： 4

Volume： 3

Page： 1853-1857

4 . 7 0 0

JCR@2023

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 10

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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