Tissue　engineering　is　a　promising　strategy　for　the　repair　of　large-scale　bone　defects,　in　which　scaffolds　and　growth　factors　are　two　critical　issues　influencing　the　efficacy　of　bone　regeneration.　Unfortunately,　the　broad　application　of　growth　factors　is　limited　by　their　poor　stability　in　the　scaffolds.　In　the　present　study,　the　strictly　controlled　expression　of　human　bone　morphogenetic　protein-4　(hBMP-4)　in　the　presence　of　doxycycline　is　achieved　by　adding　an　hBMP-4　gene　fragment　into　a　non-viral　artificial　restructuring　plasmid　vector　(pSTAR)　to　form　the　pSTAR-hBMP-4　plasmid　(phBMP-4).　Furthermore,　the　controlled　release　of　phBMP-4　is　obtained　with　an　electroactive　tissue　engineering　scaffold,　generated　by　combining　a　triblock　copolymer　of　poly(L-lactic　acid)-block-aniline　pentamer-block-poly(L-lactic　acid)　(PLA-AP)　with　poly(lactic-co-glycolic　acid)/hydroxyapatite　(PLGA/HA).　This　PLGA/HA/PLA-AP/phBMP-4　composite　scaffold,　with　controlled　gene　release　and　Dox-regulated　gene　expression　upon　electrical　stimulation,　operating　synergistically,　exhibits　an　improved　cell　proliferation　ability,　enhanced　osteogenesis　differentiation　in　vitro,　and　effective　bone　healing　in　vivo　in　a　rabbit　radial　defect　model.　Taking　these　results　together,　the　proposed　smart　PLGA/HA/PLA-AP/phBMP-4　scaffold　lays　a　solid　theoretical　and　experimental　basis　for　future　applications　of　such　multi-functional　materials　in　bone　tissue　engineering　to　help　patients　in　need.　©　2019　Elsevier　Ltd