Superwettable　patterns　with　superhydrophobic　and　superhydrophilic　units　have　the　capacity　of　enriching　and　absorbing　microdroplets　for　multifunctional　biosensing.　Combining　the　advantages　of　superwettable　micropatterns　and　a　rapid　click　reaction,　we　first　prepared　a　film　using　propargyl　methacrylate-ethylene　dimethacrylate　and　then　created　a　superhydrophobic-superhydrophilic　micropattern　by　a　rapid　thiol-yne　click　reaction.　Due　to　the　high　wettability　contrast,　water　droplets　tend　to　be　anchored　in　the　superhydrophilic　region.　Molecules　dissolved　in　a　water　droplet　are　therefore　uniformly　enriched　in　the　superhydrophilic　region　after　evaporation　because　of　the　Malangoni　effect.　This　provides　a　rational　strategy　to　develop　novel　patterned　microchips　for　sensing　applications.　Combining　with　fluorescence　imaging　technology,　the　Ti　superwettable　microchip　can　be　used　to　detect　o-phthalaldehyde　in　water,　with　a　detection　limit　as　low　as　10-7　mol　L-1.　In　addition,　taking　advantage　of　the　oxidative　color　rendering　ability　of　glucose,　the　microchip,　when　fabricated　on　a　glass　substrate,　can　realize　reuseable　glucose　detection　with　a　detection　limit　of　2　mM　within　15　min.　©　2019　American　Chemical　Society.