The　temperature　control　in　the　traditional　DNAzyme-based　electrochemiluminescent　(ECL)　biosensors　for　Pb2+　is　realized　through　bulk　solution　heating,　which　needs　tedious　procedures,　relative　large　equipment　and　this　limits　their　application.　Moreover,　the　ECL　indicators　had　been　modified　on　the　DNA　through　chemical　reaction,　which　has　the　limitation　of　low　efficiency　and　high　cost.　In　this　study,　electrically　heated　indium-tin-oxide　(ITO)　electrode　is　applied　to　adjust　the　temperature　instead　of　the　whole　bulk　solution　heating.　Y-shaped　double　strand　DNA　(dsDNA)　was　formed　through　the　hybridization　of　DNAzyme,　substrate　and　capture　DNA,　then　Ru(phen)32+　was　embedded　into　the　groove　of　dsDNA　and　acted　as　the　ECL　indicator,　which　avoided　the　costly　labeling　procedure　of　ECL　indicator　on　DNA.　The　results　indicated　that　the　two　different　ways　of　heating　had　the　same　effects　on　the　DNA　hybridization　and　DNAzyme　action,　but　the　temperature　controlled　by　the　heated　electrode　is　simpler　and　quicker　than　that　with　bulk　solution　heating.　Furthermore,　the　performance　of　the　biosensor　had　been　further　improved　at　an　elevated　electrode　surface　temperature　because　temperature　affected　the　performance　of　the　ECL　of　Ru(phen)32+　greatly.　Under　the　optimized　conditions,　the　ECL　signal　has　a　linear　relationship　with　logarithm　concentration　of　Pb2+　in　the　range　of　0.25　˜　500　pM　with　a　detection　limit　of　0.2　pM　at　the　electrode　temperature　of　45　°C,　and　this　outcome　was　approximately　5　times　lower　than　that　at　25　°C　of　electrode　surface　temperature.　The　proposed　biosensor　has　been　applied　to　detect　Pb2+　in　soil　samples　with　satisfactory　results.　©　2019　Elsevier　B.V.