The　optimization　of　the　extraction　process　of　protein　from　Ganoderma　lucidum　was　achieved　by　orthogonal　experiment　method　and　its　antioxidant　activity　were　characterized.　Ganoderma　lucidum　protein　extraction　rate　was　taken　as　the　index　to　study　the　effect　of　ratio　of　material:　liquid　(m/V),　extraction　time,　temperature　and　pH　on　extraction　yield.　On　the　basis　of　single　factor　experiment,　using　L9(34)　orthogonal　design　and　analysis　method　was　taken　to　determine　the　Ganoderma　lucidum　protein　extraction　process.　The　results　showed　that　a　material:　liquid(m/V)　of　1:120,　a　extraction　temperature　of　65　,　a　pH　of　10.0　a　pH　were　optimal　for　protein　extraction　from　Ganoderma　lucidum　and　the　extraction　ratio　was　50.52%.　The　concentration　for　50%　of　maximal　effect(EC50)　of　Ganoderma　lucidum　protein　extracted　under　the　optimal　condition　to　DPPH　free　radicals　and　hydroxyl　radicals　were　68　502　μg/mL　and　568.82　μg/mL,　ABTS　free　radical　scavenging　rate　and　metal　chelating　rate　in　the　200　μg/mL　of　99.60%　and　83.15%　respectively,　and　has　stronger　reducing　power,　which　showed　that　protein　extraction　from　Ganoderma　lucidum　has　certain　antioxidant　activity.　©　2018,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.