A　simple　and　sensitive　quartz　crystal　microbalance　(QCM)　immunosensing　platform　was　designed　for　the　high-efficient　detection　of　aflatoxin　B1　(AFB1)　in　foodstuff.　Initially,　phenoxy-derived　dextran　molecule　was　immobilized　on　the　surface　of　QCM　gold　substrate　by　using　thiolated　β-cyclodextrin　based　on　the　supramolecular　host-guest　chemistry　between　phenoxy　group　and　cyclodextrin.　Then,　AFB1-bovine　serum　albumin　(AFB1-BSA)-conjugated　concanavalin　A　(Con　A)　was　assembled　onto　the　QCM　probe　through　the　dextran-Con　A　interaction.　Glucose-loaded　nanoliposome,　labeled　with　monocolonal　anti-AFB1　antibody,　was　used　for　the　amplification　of　QCM　signal.　Upon　target　AFB1　introduction,　the　analyte　competed　with　the　immobilized　AFB1-BSA　on　the　probe　for　the　labeled　anti-AFB1　antibody　on　the　nanoliposome.　Based　on　specific　antigen-antibody　reaction,　the　amount　of　the　conjugated　nanoliposomes　on　the　QCM　probe　gradually　decreased　with　the　increment　of　target　AFB1　in　the　sample.　Upon　injection　of　Triton　X-100　in　the　detection　cell,　the　carried　nanoliposome　was　lysed　to　release　the　encapsulated　glucose　molecules.　Thanks　to　the　stronger　affinity　of　Con　A　toward　glucose　than　that　of　dextran,　AFB1-BSA-labeled　Con　A　was　displaced　from　the　QCM　probe,　resulting　in　the　change　of　the　local　frequency.　Under　the　optimum　conditions,　the　shift　of　the　functionalized　QCM　immunosensing　interface　in　the　frequency　shift　was　proportional　to　the　concentration　of　target　AFB1　within　a　dynamic　range　from　1.0　ng　kg−1　to　10　μg　kg-1　at　a　low　detection　limit　of　0.83　ng　kg−1.　In　addition,　the　acceptable　assayed　results　on　precision,　reproducibility,　specificity　and　method　accuracy　for　the　analysis　of　real　samples　were　also　acquired.　Importantly,　our　strategy　can　provide　a　signal-on　competitive　immunoassay　for　the　detection　of　small　molecules,　e.g.,　mycotoxins　and　biotoxins,　thereby　representing　a　versatile　sensing　schemes　by　controlling　the　corresponding　antibody　or　hapten　in　the　analysis　of　food　safety.　©　2018　Elsevier　B.V.