Aiming　at　the　uncertainty　in　the　daily　travel　decision,　this　paper　investigates　the　route　choice　behavior　under　the　condition　of　two　reference　points　based　on　the　prospect　theory.　First　of　all,　the　route　choice　model　based　on　the　prospect　theory　is　proposed　by　taking　the　acceptable　earliest　arrival　time　and　the　working　start　time　as　the　two　reference　points.　Then,　a　questionnaire　is　designed　and　the　results　of　the　route　choice　in　the　four　scenarios　are　counted.　Using　the　nonlinear　regression　method,　we　estimate　the　parameters　of　the　value　function　and　the　weight　function　of　the　two　reference　points＇route　choice　model.　Furthermore,　the　parameter　estimation　results　are　compared　with　the　parameters　estimated　in　the　previous　literature.　The　results　show　that　the　parameter　values　obtained　in　the　case　of　single　reference　point　do　not　apply　to　the　case　of　two　reference　points.　At　the　same　time,　it　is　determined　that　the　value　function　is　an　asymmetric　curve,　and　the　expected　arrival　time　is　close　to　the　working　start　time.　The　slope　of　the　value　function　curve　is　steeper　in　the　late　arrival　gain　interval.　Copyright　©　2017　by　Science　Press.