A　novel　sodium　hyaluronate-functionalized　urea-formaldehyde　(UF)　monolithic　column　has　been　developed　by　in-situ　polycondensation　of　urea,　formaldehyde　and　sodium　hyaluronate　(HA).　HA　plays　both　the　roles　of　crosslinking　and　hydrophilic　functionalization.　The　preparation　factors　including　different　molecular　weights　of　HA　and　different　amounts　of　HA　were　optimized,　and　then　a　uniform　monolith　with　satisfactory　permeability　and　hydrophilic　binding　capacity　was　obtained.　Due　to　the　excellent　hydrophilicity　of　HA,　HA-functionalized　UF　monolith　showed　higher　hydrophilic　extraction　efficiency　than　UF　monolith,　and　was　applied　for　hydrophilic　in-tube　solid-phase　microextraction　(SPME)　of　melamine　(MEL).　Several　factors　for　hydrophilic　in-tube　SPME,　such　as　ACN　percentage　in　the　sampling　solution,　salt　concentration　and　pH　value　of　the　sampling　solution,　elution　volume,　sampling　and　elution　flow　rate,　were　investigated　with　respect　to　the　extraction　efficiency　of　MEL.　Under　the　optimized　SPME　conditions,　the　limit　of　detection　(LOD)　of　MEL　was　found　to　be　0.2　ng/mL　in　the　milk　formula　samples,　the　recoveries　of　MEL　spiked　in　milk　formula　samples　ranged　from　87.3%　to　96.7%　with　relative　standard　deviations　(RSDs)　less　than　5.1%.　Owing　to　the　excellent　hydrophilic　extraction　ability,　the　novel　HA-functionalized　UF　monolith　could　provide　a　promising　tool　for　the　sensitive　analysis　of　polar　analytes　in　complicated　samples.　©　2017　Elsevier　B.V.