In　order　to　prepare　hybrid　nanostructure　composite　with　magnetocaloric　effect,　combining　with　the　superior　characteristics　of　chitosan　(CS)　and　collagen　(Col)　as　the　organic　matrix,　the　soluble　calcium　salt　and　soluble　phosphate　as　the　precursor　of　inorganic　phase　nano　hydroxyapatite　(nHAP),　and　the　soluble　salt　and　soluble　ferrous　salt　as　precursor　to　inorganic　phase　superparamagnetic　nano　Fe3O4　particles　were　used　to　prepare　Fe3O4-CS-Col-nHAP　composite　scaffold　by　in　situ　fabrication　and　freeze-drying　technique.　The　composition　and　morphology　of　Fe3O4-CS-Col-nHAP　composite　scaffold　and　the　distribution　of　nano　particles　were　characterized　by　FTIR　spectroscopy,　XRD　and　SEM.　And　the　magnetic　energy　of　the　scaffold　was　tested　by　physical　property　tester　(PPMS).　The　results　show　that　Fe3O4-CS-Col-nHAP　composite　scaffold　presents　a　homogeneously　interconnected　macroporous　structure　which　has　an　everage　size　of　around　100-150　μm　and　the　porosity　is　about　95%.　And　the　low　crystallinity　of　nHAP　crystals　and　nano　Fe3O4　particles　are　uniformly　distributed　in　the　organic　matrix.　The　nano　Fe3O4　particles　prepared　by　in　situ　fabrication　has　super　paramagnetic　properties,　with　the　increase　of　magnetic　nano　particles,　the　magnetic　saturation　strength　is　enhanced.　The　saturation　magnetization　intensionsity　of　Fe3O4-CS-Col-nHAP　composite　scaffold　is　about　0.025　emu/g.　Therefore,　the　hybrid　nanostructure　Fe3O4-CS-Col-nHAP　composite　scaffold　prepared　by　in　situ　fabrication　and　freeze-drying　technique　has　good　magnetocaloric　effect,　and　is　promising　for　bone　repair　application　in　bone　tissue　engineering.　©　2017,Chinese　Society　for　Composite　Materials.　All　right　reserved.