Removal　of　Pb2 +　and　biodegradation　of　organophosphorus　have　been　both　widely　investigated　respectively.　However,　bio-remediation　of　both　Pb2 +　and　organophosphorus　still　remains　largely　unexplored.　Bacillus　subtilis　FZUL-33,　which　was　isolated　from　the　sediment　of　a　lake,　possesses　the　capability　for　both　biomineralization　of　Pb2 +　and　biodegradation　of　acephate.　In　the　present　study,　both　Pb2 +　and　acephate　were　simultaneously　removed　via　biodegradation　and　biomineralization　in　aqueous　solutions.　Batch　experiments　were　conducted　to　study　the　influence　of　pH,　interaction　time　and　Pb2 +　concentration　on　the　process　of　removal　of　Pb2 +.　At　the　temperature　of　25°C,　the　maximum　removal　of　Pb2 +　by　B.　subtilis　FZUL-33　was　381.31 ± 11.46 mg/g　under　the　conditions　of　pH 5.5,　initial　Pb2 +　concentration　of　1300 mg/L,　and　contact　time　of　10 min.　Batch　experiments　were　conducted　to　study　the　influence　of　acephate　on　removal　of　Pb2 +　and　the　influence　of　Pb2 +　on　biodegradation　of　acephate　by　B.　subtilis　FZUL-33.　In　the　mixed　system　of　acephate–Pb2 +,　the　results　show　that　biodegradation　of　acephate　by　B.　subtilis　FZUL-33　released　PO43 +,　which　promotes　mineralization　of　Pb2 +.　The　process　of　biodegradation　of　acephate　was　affected　slightly　when　the　concentration　of　Pb2 +　was　below　100 mg/L.　Based　on　the　results,　it　can　be　inferred　that　the　B.　subtilis　FZUL-33　plays　a　significant　role　in　bio-remediation　of　organophosphorus-heavy　metal　compound　contamination.　©　2016