A　new　method　for　electrochemical　detection　of　DNA　hybridization　with　elevated　electrode　temperature　at　gold　particles　modified　heated　gold　disk　electrode　(Au-HAuDE)　is　demonstrated.　This　method　employs　a　hairpin　DNA　probe　for　target　DNA　capture　and　incorporates　a　specific　DNAzyme　sequence　into　the　probe　as　electrocatalytic　label.　The　hairpin　DNA　probes　are　immobilized　on　the　Au-HAuDE.　The　presence　of　target　DNA　opens　the　hairpin　structure,　releases　the　DNAzyme　sequence,　and　forms　the　hemin/G-quadruplex　peroxidase-mimicking　DNAzyme　(HRP-DNAzyme)　with　hemin　addition.　With　elevated　electrode　temperature,　the　electrocatalytic　activity　of　HRP-DNAzyme　for　H2O2　reduction　by　oxidation　of　hydroquinone　(used　as　an　electron　transfer　mediator)　can　be　significantly　improved,　resulting　in　an　enhanced　electrochemical　reduction　current　of　benzoquinone　for　DNA　detection.　With　an　electrode　temperature　of　50　°C,　the　detection　limit　for　target　DNA　measurements　can　be　decreased　ca.　one　magnitude　compared　with　that　at　0　°C.　©　2015　Elsevier　B.V.　All　rights　reserved.