A new formula to calculate activity of superoxide dismutase in indirect assays - Details

author：

Zhang, Chen (Zhang, Chen.) ^[1] | Bruins, Marieke E. (Bruins, Marieke E..) ^[2] | Yang, Zhi-Qiang (Yang, Zhi-Qiang.) ^[3] | Liu, Shu-Tao (Liu, Shu-Tao.) ^[4] | Rao, Ping-Fan (Rao, Ping-Fan.) ^[5]

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Abstract：

To　calculate　superoxide　dismutase　(SOD)　activity　rapidly　and　accurately　by　indirect　SOD　assays,　a　formula　based　on　the　ratio　of　the　catalytic　speed　of　SOD　to　the　reaction　speed　of　the　indicator　with　superoxide　anion　was　deduced.　The　accuracy　of　this　formula　was　compared　with　the　conventional　formula　based　on　inhibition　in　five　indirect　SOD　assays.　The　new　formula　was　validated　in　nearly　the　entire　SOD　activity　range,　whereas　the　conventional　formula　was　validated　only　during　inhibition　of　40-60%.　This　formula　might　also　be　used　for　the　assays　of　other　enzymes.　©　2016　Elsevier　Inc.　All　rights　reserved.

Keyword：

Amines Enzymes

Community：

[ 1 ] [Zhang, Chen]Institute of Biotechnology, Fuzhou University, Fuzhou; Fujian; 350002, China
[ 2 ] [Zhang, Chen]Biobased Chemistry and Technology, Wageningen; 6708 WG, Netherlands
[ 3 ] [Bruins, Marieke E.]Wageningen UR Food and Biobased Research, Wageningen; 6708 WG, Netherlands
[ 4 ] [Yang, Zhi-Qiang]Institute of Biotechnology, Fuzhou University, Fuzhou; Fujian; 350002, China
[ 5 ] [Liu, Shu-Tao]Institute of Biotechnology, Fuzhou University, Fuzhou; Fujian; 350002, China
[ 6 ] [Rao, Ping-Fan]CAS.SIBS, Zhejiang Gongshang University, Joint Center for Food and Nutrition Research, Hangzhou; 310035, China

Reprint 's Address：

[liu, shu-tao]institute of biotechnology, fuzhou university, fuzhou; fujian; 350002, china

Email：

stliu@fzu.edu.cn

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Source ：

Analytical Biochemistry

ISSN： 0003-2697

Year： 2016

Volume： 503

Page： 65-67

2 . 3 3 4

JCR@2016

2 . 6 0 0

JCR@2023

ESI HC Threshold：253

JCR Journal Grade：2

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 34

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 1

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