An　amperometric　glucose　biosensor　based　on　direct　electron　transfer　of　glucose　oxidase　(GOD)　self-assembled　on　the　surface　of　partially　unzipped　carbon　nanotubes　(PUCNTs)　modified　glassy　carbon　electrode　(GCE)　has　been　successfully　fabricated.　PUCNTs　were　synthesized　via　a　facile　chemical　oxidative　etching　CNTs　and　used　as　a　novel　immobilization　matrix　for　GOD.　The　cyclic　voltammetric　result　of　the　PUCNT/GOD/GCE　showed　a　pair　of　well-defined　and　quasi-reversible　redox　peaks　with　a　formal　potential　of-0.470　V　and　a　peak　to　peak　separation　of　37　mV,　revealing　that　the　fast　direct　electron　transfer　between　GOD　and　the　electrode　has　been　achieved.　It　is　notable　that　the　glucose　determination　has　been　achieved　in　mediator-free　condition.　The　developed　biosensor　displayed　satisfactory　analytical　performance　toward　glucose　including　high　sensitivity　(19.50　μA　mM-1　cm-2),　low　apparent　Michaelis-Menten　(5.09　mM),　a　wide　linear　range　of　0-17　mM,　and　also　preventing　the　interference　from　ascorbic　acid,　uric　acid　and　dopamine　usually　coexisting　with　glucose　in　human　blood.　In　addition,　the　biosensor　acquired　excellent　storage　stabilities.　This　facile,　fast,　environment-friendly　and　economical　preparation　strategy　of　PUCNT-GOD　may　provide　a　new　platform　for　the　fabrication　of　biocompatible　glucose　biosensors　and　other　types　of　biosensors.　©　2015　Elsevier　B.V.　All　rights　reserved.