Objective:　The　main　purpose　of　this　research　is　isolation,　purification　and　partial　characterization　of　a　lectin　from　black　kidney　beans.　Methods:　The　isolation　and　purification　procedure　contained　buffer　extraction,　affinity　chromatography　on　AF-Blue　HC-650M,　ion-exchange　chromatography　on　CM　Sephadex　c-25,　gel　filtration　chromatography　on　Sephadex　G-50.　SDS-PAGE　was　used　to　identify　its　purity　and　analyze　the　molecular　weight,　and　the　partial　biological　function　activity　of　the　lectin　including　hemagglutinating　activity　and　antifungal　activity　had　also　been　studied.　Results:　A　lectin　was　isolated　and　purified　from　Black　kidney　beans,　named　BKBL.　The　lectin　was　a　purified　monomelic　protein　with　a　molecular　weight　of　36　ku　by　SDS-PAGE,　and　was　also　identified　as　a　glycoprotein　with　11.9%　sugar　content　through　PAS　dyeing.　BKBL　exhibited　hemagglutinating　activity　in　both　human　erythrocytes　and　mice　erythrocytes,　and　the　effect　was　more　obvious　in　the　latter.　The　hemagglutinating　activity　was　not　effected　below　70　and　between　pH　2~10,　its　activity　remained　50%　after　being　treated　with　pH　11　for　30　min,　but　it　would　lose　its　activity　at　100　treatment　for　30　min.　BKBL　showed　antifungal　activity　towards　the　growth　of　Aspergillus　niger,　Fusarium　oxysporum　f.sp.　cubense,　Colletotrichum　gloeosporioides　Penz,　Alternaria　tenuissima,　Pythium　aphanidermatum,　Botrytis　cinerea.　Conclusion:　A　lectin　can　be　isolated　and　purified　from　Black　kidney　beans,　with　strong　and　stable　hemagglutinating　activity　towards　mice　erythrocytes,　and　antifungal　activity　towards　most　fungi.　©,　2015,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.