Fluorescence probe techniques to study the interaction between hydroxylated polybrominated diphenyl ethers (OH-PBDEs) and protein disulfide isomerase (PDI) - Details

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Abstract：

A　selective　and　sensitive　method　to　study　the　interaction　between　hydroxylated　polybrominated　diphenyl　ethers　(OH-PBDEs)　and　protein　disulfide　isomerase　(PDI)　was　established　in　this　assay.　In　this　report,　3,3′,5-triiodo-thyronine　(T3)　was　conjugated　with　fluorescein　(FITC)　as　a　fluorescence　probe　(F-T3)　to　study　the　competitive　binding　interaction　of　OH-PBDEs　to　PDI,　which　was　observed　to　exhibit　T3　binding　activity.　The　findings　suggest　that　some　OH-PBDEs　have　the　potential　of　binding　to　PDI,　and　they　share　the　same　binding　site　as　T3　to　PDI;　moreover,　OH-PBDEs　were　able　to　act　as　a　competitive　inhibitor　in　PDI　binding　to　T3.　©　the　Partner　Organisations　2014.

Keyword：

Binding energy Ethers Fluorescence Hydroxylation Organic pollutants Probes Proteins Sulfur compounds

Community：

[ 1 ] [He, Xiaoyan]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou Fujian; 350002, China
[ 2 ] [Zhang, Huimin]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou Fujian; 350002, China
[ 3 ] [Wu, Jun]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou Fujian; 350002, China
[ 4 ] [Qiu, Bin]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou Fujian; 350002, China
[ 5 ] [Qiu, Bin]Department of Chemistry, Hong Kong Baptist University, Hong Kong, Hong Kong
[ 6 ] [Lin, Zhenyu]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou Fujian; 350002, China
[ 7 ] [Chen, Guonan]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou Fujian; 350002, China
[ 8 ] [Cai, Zongwei]Department of Chemistry, Hong Kong Baptist University, Hong Kong, Hong Kong

Reprint 's Address：

[qiu, bin]department of chemistry, hong kong baptist university, hong kong, hong kong;;[qiu, bin]ministry of education, key laboratory of analysis and detection for food safety, fuzhou university, fuzhou fujian; 350002, china

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Source ：

Analytical Methods

ISSN： 1759-9660

Year： 2014

Issue： 20

Volume： 6

Page： 8106-8109

1 . 8 2 1

JCR@2014

2 . 7 0 0

JCR@2023

ESI HC Threshold：268

JCR Journal Grade：2

CAS Journal Grade：3

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WoS CC Cited Count： 0

SCOPUS Cited Count： 3

ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 0

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