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author:

Qiu, Bin (Qiu, Bin.) [1] | zhang, Ya-shan (zhang, Ya-shan.) [2] | Lin, Yi-bing (Lin, Yi-bing.) [3] | Lu, Yu-Jing (Lu, Yu-Jing.) [4] | Lin, Zhen-yu (Lin, Zhen-yu.) [5] | Wong, Kwok-Yin (Wong, Kwok-Yin.) [6] | Chen, Guo-nan (Chen, Guo-nan.) [7]

Indexed by:

EI

Abstract:

In this paper, we reported a convenient fluorescence method for the detection of genetically modified organisms (GMOs). As it is known that the cauliflower mosaic virus (CaMV) 35S promoter is widely used in most transgenic plants (Schnurr and Guerra, 2000), we thus design a simple method based on the detection of a section target DNA (DNA-T) from the transgene CaMV 35S promoter. In this method, the full-length guanine-rich single-strand sequences were split into fragments (Probe 1 and 2) and each part of the fragment possesses two GGG repeats. In the presence of K+ ion and berberine, if a complementary target DNA of the CaMV 35S promoter was introduced to hybridize with Probe 1 and 2, a G-quadruplex-berberine complex was thus formed and generated a strong fluorescence signal. The generation of fluorescence signal indicates the presence of CaMV 35S promoter. This method is able to identify and quantify Genetically Modified Organisms (GMOs), and it shows wide linear ranges from 5.0×10-9 to 9.0×10-7mol/L with a detection limit of 2.0×10-9mol/L. © 2012 Elsevier B.V.

Keyword:

Biosensors DNA Fluorescence Probes Viruses

Community:

  • [ 1 ] [Qiu, Bin]Ministry of Education Key Lab. of Analysis and Detection Technology for Food Safety Fuzhou Univ., Department of Chemistry, Fuzhou University, Fuzhou 350002, China
  • [ 2 ] [Qiu, Bin]The State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, Hong Kong
  • [ 3 ] [zhang, Ya-shan]Ministry of Education Key Lab. of Analysis and Detection Technology for Food Safety Fuzhou Univ., Department of Chemistry, Fuzhou University, Fuzhou 350002, China
  • [ 4 ] [Lin, Yi-bing]Ministry of Education Key Lab. of Analysis and Detection Technology for Food Safety Fuzhou Univ., Department of Chemistry, Fuzhou University, Fuzhou 350002, China
  • [ 5 ] [Lu, Yu-Jing]Ministry of Education Key Lab. of Analysis and Detection Technology for Food Safety Fuzhou Univ., Department of Chemistry, Fuzhou University, Fuzhou 350002, China
  • [ 6 ] [Lu, Yu-Jing]Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • [ 7 ] [Lin, Zhen-yu]Ministry of Education Key Lab. of Analysis and Detection Technology for Food Safety Fuzhou Univ., Department of Chemistry, Fuzhou University, Fuzhou 350002, China
  • [ 8 ] [Wong, Kwok-Yin]The State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR, Hong Kong
  • [ 9 ] [Chen, Guo-nan]Ministry of Education Key Lab. of Analysis and Detection Technology for Food Safety Fuzhou Univ., Department of Chemistry, Fuzhou University, Fuzhou 350002, China

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Source :

Biosensors and Bioelectronics

ISSN: 0956-5663

Year: 2013

Issue: 1

Volume: 41

Page: 168-171

6 . 4 5 1

JCR@2013

1 0 . 7 0 0

JCR@2023

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 26

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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