A　molecularly　imprinted　inorganic-organic　hybrid　monolithic　capillary　column　(MIP　hybrid　monolith)　was　synthesized　by　one-pot　process　and　its　application　in　selective　recognition　and　capture　of　lysozyme　(Lyz)　from　complex　biological　samples　was　described　for　the　first　time.　Due　to　a　combination　of　rigid　silica　matrices　and　flexible　organic　hydrogels　in　one-pot　process,　stable　and　accessible　recognition　sites　in　the　as-prepared　MIP　hybrid　monolith　could　be　obtained　after　the　removal　of　template　protein,　which　facilitated　the　rebinding　of　template　and　provided　good　reproducibility　and　lifetime　of　use.　The　morphology,　permeability,　and　pore　properties　of　the　as-prepared　MIP　hybrid　monolith　were　characterized　and　a　uniform　monolithic　matrix　with　high　surface　area　and　large　through-pores　was　observed.　The　recognition　behavior　of　MIP　and　non-imprinted　(NIP)　hybrid　monolith　was　evaluated　by　separating　template　protein　from　unfractionated　protein　mixture　and　the　result　indicated　that　the　MIP　hybrid　monolith　has　much　higher　affinity　toward　the　template　protein　than　NIP　hybrid　monolith.　High　imprinted　factor　(IF)　and　separation　efficiency　could　be　obtained.　In　addition,　the　practicality　of　the　Lyz-MIP　hybrid　monolith　was　further　evaluated　by　selective　separation　of　Lyz　from　egg　white　and　capture　of　Lyz　from　human　serum　by　adopting　it　as　an　in-tube　solid　phase　microextraction　(in-tube　SPME),　and　the　good　results　demonstrated　its　potential　in　proteome　analysis.　©　2013　Elsevier　B.V.