This　paper　describes　the　use　of　QuEChERS　(Quick,　Easy,　Cheap,　Effective,　Rugged　and　Safe)　for　the　extraction,　cleanup　and　detection　of　10　paralytic　shellfish　toxins　(PSP)　in　sea　food　by　HILIC-MS/MS　with　positive　ESI.　Matrix　matched　calibration　standards　were　used　to　compensate　for　matrix　effects.　The　toxins　were　extracted　with　acetonitrile/water　(90:10,　v/v)　containing　0.1%　formic　acid　and　cleaned　by　HLB　and　GCB　sorbents.　Qualitative　and　quantitative　detection　for　the　analytes　were　carried　out　under　the　multiple　reaction　monitoring　(MRM)　in　positive　ionization　mode　after　chromatography　separation　on　a　TSK-gel　Amide-80®　column　(150　mm　×　2.0　mm　×　3　μm).　Studies　at　three　fortification　levels　for　the　toxins　in　the　range　of　8.1-225.5　μg/kg　gave　mean　recoveries　from　71.3%　to　104.6%　with　relative　standard　deviation　(RSD)　≤　15.8%.　The　limit　of　detection　(LOD)　was　below　the　recommended　regulatory　limit　of　170　μg　STXequ./kg　and　the　proposed　method　fully　meets　the　needs　of　daily　monitoring.　©　2012　Elsevier　Ltd.　All　rights　reserved.