As　long-span　cable-stayed　bridge　with　multi-tower　has　complex　mechanical　characteristics,　the　seismic　response　is　quite　different　when　the　lightweight　composite　girder　is　adopted.　In　this　paper,　Wuhan　Yangtze　River　Bridge　is　taken　as　background　project　and　test　mode　of　multi-tower　cable-stayed　bridge　is　designed.　Based　on　shaking　table　test　of　Fuzhou　University,　the　seismic　response　laws　of　long-span　cable-stayed　bridge　under　uniform　excitation　and　non-uniform　excitation　were　discussed　respectively.　The　dynamic　property　identified　from　recorded　data　shows　the　longitudinal　stiffness　of　middle　towers　is　relatively　small.　Results　of　shaking　table　test　show　that　the　tower　and　girder　with　greater　seismic　response　and　wave　propagation　effects　on　seismic　response　can　not　be　ignored.