Increasing the sensitivity and single-base mismatch selectivity of the molecular beacon using graphene oxide as the 'nanoquencher' - Details

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Abstract：

Here,　we　report　a　novel,　highly　sensitive,　selective　and　economical　molecular　beacon　using　graphene　oxide　as　the＇nanoquencher＇.　This　novel　molecular　beacon　system　contains　a　hairpin-structured　fluorophorelabeled　oligonucleotide　and　a　graphene　oxide　sheet.　The　strong　interaction　between　hairpin-structured　oligonucleotide　and　graphene　oxide　keep　them　in　close　proximity,　facilitating　the　flu´ores-cence　quenching　of　the　fluorophore　by　graphene　oxide.　In　the　presence　of　a　complementary　target　DNA,　the　binding　between　hairpin-structured　oligonucleotide　and　target　DNA　will　disturb　the　interaction　between　hairpin-struc-tured　oligonucleotide　and　graphene　oxide,　and　release　the　oligonucleotide　from　graphene　oxide,　resulting　in　restoration　of　fluorophore　fluorescence.　In　the　present　study,　we　show　that　this　novel　graphene　oxide　quenched　molecular　beacon　can　be　used　to　detect　target　DNA　with　higher　sensitivity　and　single-base　mismatch　selectivity　compared　to　the　conventional　molecular　beacon.　©　2010　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA,.

Keyword：

Biosensors DNA Fluorophores Graphene Oligonucleotides

Community：

[ 1 ] [Lu, Chun-Hua]Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, 350002 Fuzhou, China
[ 2 ] [Li, Juan]Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, 350002 Fuzhou, China
[ 3 ] [Liu, Jing-Jing]Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, 350002 Fuzhou, China
[ 4 ] [Yang, Huang-Hao]Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, 350002 Fuzhou, China
[ 5 ] [Chen, Xi]State Key Laboratory of Marine Environmental Science, Xiamen University, 361005 Xiamen, China
[ 6 ] [Chen, Guo-Nan]Key Laboratory of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry and Chemical Engineering, Fuzhou University, 350002 Fuzhou, China

Reprint 's Address：

Email：

hhyang@fzu.edu.cn

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Source ：

Chemistry - A European Journal

ISSN： 0947-6539

Year： 2010

Issue： 16

Volume： 16

Page： 4889-4894

5 . 4 7 6

JCR@2010

3 . 9 0 0

JCR@2023

JCR Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 178

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 4

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