A　poly(nickel(II)　tetrasulfophthalocyanine)/multi-walled　carbon　nanotubes　composite　modified　electrode　(polyNiTSPc/MWNTs)　was　fabricated　by　electropolymerization　of　NiTSPc　on　MWNTs-modified　glassy　carbon　electrode　(GCE).　The　modified　electrode　was　found　to　be　able　to　greatly　improve　the　emission　of　luminol　electrochemiluminescence　(ECL)　in　a　solution　containing　hydrogen　peroxide.　Glucose　oxidase　(GOD)　was　immobilized　on　the　surface　of　polyNiTSPc/MWNTs　modified　GC　electrode　by　Nafion　to　establish　an　ECL　glucose　sensor.　Under　the　optimum　conditions,　the　linear　response　range　of　glucose　was　1.0　×　10-6　to　1.0　×　10-4　mol　L-1　with　a　detection　limit　of　8.0　×　10-8　mol　L-1　(defined　as　the　concentration　that　could　be　detected　at　the　signal-to-noise　ratio　of　3).　The　ECL　sensor　showed　an　outstanding　well　reproducibility　and　long-term　stability.　The　established　method　has　been　applied　to　determine　the　glucose　concentrations　in　real　serum　samples　with　satisfactory　results.　©　2008　Elsevier　B.V.　All　rights　reserved.