An　electrochemiluminescent　(ECL)　enzyme　biosensor　was　developed　for　detection　of　hypoxanthine　based　on　ECL　of　6-(4-methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one　(MCLA).　The　xanthine　oxidase　(XOD)　was　modified　on　the　heated　indium-tin-oxide　(ITO)　electrode,　and　the　ECL　enzyme　biosensor　was　found　to　be　electrode　temperatures　depend,　this　was　because　that　both　the　diffusion　and　convection　of　compounds　near　the　electrode　surface　and　the　catalytic　reaction　of　immobilized　enzyme　were　affected　by　the　temperature　of　electrode　(Te).　In　this　work,　the　highest　sensitivity　was　gained　when　Te　was　36　°C.　Under　the　optimum　condition,　the　linear　response　range　of　hypoxanthine　(HX)　was　found　to　be　0.3-27　μmol/L,　and　the　detection　limit　(defined　as　the　concentration　that　could　be　detected　at　the　signal-to-noise　ration　of　3)　was　0.15　μmol/L.　©　2009　Elsevier　B.V.　All　rights　reserved.