The　synthesis　of　poly-aminophenylboronic　acid　(APBA)　imprinted　hybrid　silica-based　polymers　for　selective　recognition　of　bovine　hemoglobin　(BHb)　was　described,　where　the　mesoporous　hybrid　silica　supporting　matrices　were　prepared　by　a　mild　sol-gel　process　with　tetraethoxysilane　and　3-　aminopropyltriethoxysilane　as　two　precursors.　Covalent　immobilization　of　BHb　was　adopted　in　order　to　create　homogeneous　recognition　sites.　After　removal　of　the　template,　the　resulting　imprinted　polymers　showed　high　binding　affinity　toward　BHb　and　the　imprinting　factor　(α)　reached　2.12.　The　specificity　of　the　BHb　recognition　was　evaluated　with　competitive　experiments,　indicating　the　imprinted　polymers　have　a　higher　selectivity　for　the　template　BHb.　The　easy　preparation　protocol　and　good　protein　recognition　properties　made　the　approach　an　attractive　solution　to　depletion　of　high-abundance　protein　from　bovine　blood.　©　2009　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA.