A　novel　silica　monolithic　stationary　phase　functionalized　with　3-(2-aminoethylamino)　propyl　ligands　for　pressurized　CEC　has　been　presented.　The　monolithic　capillary　columns　were　prepared　by　a　sol-gel　process　in　75　pm　id　fused-silica　capillaries　and　followed　by　a　chemical　modification.　The　diamino　groups　on　the　surface　of　the　stationary　phase　are　meant　to　generate　the　chromatographic　surface　and　a　substantial　anodic　EOF　as　well　as　to　provide　electrostatic　interaction　sites　for　charged　solutes.　The　electrochromatographic　characterization　and　column　performance　were　evaluated　by　a　variety　of　neutral　and　charged　solutes.　It　was　observed　that　the　anodic　EOF　for　the　diamine-bonded　monolith　was　greatly　affected　by　the　reaction　time　with　3-(2-aminoethylamino)propyltrimethoxysilane　and　the　PEG　amount　in　the　solgel　reaction　mixture　in　addition　to　the　mobile　phase　conditions.　The　monolithic　stationary　phase　exhibited　hydrophilic　interaction　chromatographic　behavior　toward　neutral　solutes.　Good　separations　of　various　solutes　including　phenols,　nucleic　acid　bases,　nucleosides　and　nucleotides　were　achieved　under　different　experimental　conditions.　Fast　and　efficient　separations　were　obtained　with　high　plate　counts　reaching　more　than　130　000　plates/m.　©　2008　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim.