The　flexural　fatigue　performance　of　polyacrylonitrile　(PAN)　fiber　reinforced　concrete　(PANFRC)　was　investigated　by　third-point　loading　tests.　Based　on　the　previous　research　work,　optimum　mixture　proportions　of　PANFRC　for　highway　overlays　and　bridge　decks　that　satisfied　both　the　minimum　compressive　and　bending　strengths,　and　showed　excellent　mechanical　properties,　were　selected　for　fatigue　testing.　The　experimental　program　included　a　total　of　69　flexural　specimens,　15　of　which　were　plain　concrete　specimens,　and　the　remaining　54　specimens　were　PANFRC　specimens.　Three　mixes　containing　0.0%,　0.1　%,　and　0.15%　of　PAN　fiber　volume　fractions　were　selected.　For　each　mix,　4　different　target　load　ranges　were　applied:　10-75%,　10-80%,　10-85%,　and　10-90%　of　the　ultimate　flexural　capacity,　as　obtained　from　the　corresponding　control　static　test.　The　bending　fatigue　life　of　PANFRC　specimens　under　various　stress　ratios　are　proved　to　follow　two-parameter　Weibull　distribution.　Both　a　semi-logarithm　and　a　double-logarithm　P-S-N　equations　with　various　failure　probabilities　are　derived　from　the　experimental　measurements.　The　denifition　of　the　fatigue　damage　variable　and　damage　evolution　equation　for　PANFRC　are　furtherly　proposed　based　on　theory　of　continuum　damage　mechanics.