A　scaffold　composed　of　different　collagen　(COL)/chitosan　(CS)/hyaluronic　acid　sodium　(HAS)　salt　ratios　was　evaluated　by　determining　porosity,　swelling,　loss　rate　in　hot　water,　mechanical　property,　and　cell　proliferation　to　obtain　optimum　conditions　for　manufacturing　porous　scaffolds.　Results　showed　that　the　optimal　ratio　of　COL/CS/HAS　salt　porous　scaffold　was　1:1:0.1.　High　swelling　and　loss　rate　of　scaffolds/microspheres　(MPs)　could　lead　to　high　diffusion　rate　of　MPs　from　the　scaffolds,　causing　an　increase　in　the　kartogenin　(KGN)　release.　The　porous　scaffolds　at　optimum　conditions　had　a　maximum　amount　of　KGN　release.　Results　of　in　vitro　fluorescence　staining　and　cell　proliferation　suggested　that　scaffolds/MPs　had　good　biocompatibility　and　the　capability　to　promote　bone　marrow　stromal　cell　proliferation,　cartilage　tissue　regeneration,　and　integration　between　the　repaired　and　surrounding　cartilages.　Therefore,　this　composite　could　be　a　promising　material　for　cartilage　repair　and　regeneration,　which　could　be　effective　in　the　knee　osteoarthritis　treatment.　©　2017,　©　2017　Informa　UK　Limited,　trading　as　Taylor　&　Francis　Group.