A　new　flow　injection　chemiluminescent　method　has　been　developed　for　the　determination　of　procaine　hydrochloride,　based　on　the　inhibition　of　the　chemiluminescence　reaction　of　luminol-hydrogen　peroxide　by　procaine　hydrochloride.　The　influence　of　several　surfactants　and　β-cyclodextrin　on　the　chemiluminescence　intensity　were　studied.　It　was　found　that　β-cyclodextrin　enhanced　the　decrease　in　chemiluminescence　intensity.　The　method　is　simple,　convenient　and　sensitive,　with　a　detection　limit　(3　σ)　of　0.08　μg/mL.　The　decreased　chemiluminescence　intensity　is　linear,　with　the　concentration　of　procaine　hydrochloride　in　the　range　0.2-100.0　μg/mL　and　100.0-400.0　μg/mL.　The　relative　standard　deviation　for　10　repeated　measurements　were　4.5%　and　3.4%　for　1.0　and　20.0　μg/mL　procaine　hydrochloride,　respectively.　The　method　has　been　successfully　applied　to　the　determination　of　procaine　hydrochloride　in　injection　solutions　of　this　drug.　Copyright　©　2003　John　Wiley　&　Sons,　Ltd.