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author:

Lu, W.-H. (Lu, W.-H..) [1] | Deng, W.-H. (Deng, W.-H..) [2] | Liu, S.-T. (Liu, S.-T..) [3] | Chen, T.-B. (Chen, T.-B..) [4] | Rao, P.-F. (Rao, P.-F..) [5]

Indexed by:

Scopus

Abstract:

Capillary electrophoresis (CE) of erythrocytes from different sources under various conditions is reported in this paper. It was found that erythrocyte samples from sheep, duck, and human showed characteristic and reproducible elution peaks, and that the retention times of A-, B-, AB-, and O-type erythrocytes from human blood were distinctively different; even subtle differences, among individuals with the same blood type could be detected by CE. A strictly linear correlation was obtained between the peak area and the amount of human erythrocyte over a range of 4.8×10 2 -1.9×10 4 cells (r=0.999), indicating that CE could be used for rapid and accurate quantification of erythrocytes. Using this CE protocol, the decrease of the surface electrical charge of erythrocyte during storage was confirmed. Therefore, this work demonstrated that CE could be a useful alternative for characterizing and quantifying erythrocytes or other cells. © 2003 Elsevier Science (USA). All rights reserved.

Keyword:

Capillary electrophoresis; Erythrocyte; Intact cells

Community:

  • [ 1 ] [Lu, W.-H.]Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, China
  • [ 2 ] [Deng, W.-H.]Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, China
  • [ 3 ] [Liu, S.-T.]Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, China
  • [ 4 ] [Chen, T.-B.]Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, China
  • [ 5 ] [Rao, P.-F.]Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, China

Reprint 's Address:

  • [Rao, P.-F.]Institute of Biotechnology, Fuzhou University, Gong-Ye-Ru Road 523, Fuzhou, Fujian Province 350002, China

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Source :

Analytical Biochemistry

ISSN: 0003-2697

Year: 2003

Issue: 2

Volume: 314

Page: 194-198

2 . 1 7 4

JCR@2003

2 . 6 0 0

JCR@2023

JCR Journal Grade:2

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 19

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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